A Post-doctoral position is open in the Department of Physical Chemistry at
CEA-Saclay (10-20 km near Paris).
*Duration:* 12 months (extendible)
*Starting date:* First quarter of 2019
*Financing:* French Alternative Energies and Atomic Energy Commision
à l'énergie atomique et aux énergies alternatives*).
*Project: **Development of a nuclearised instrumental platform and
associated methods **to analyse actinide-peptide species of interest in
Understanding and limiting the impact of actinides (An) generated by
research and industry on man is a major issue. In this sense, the knowledge
of the affinity of An for their target proteins could help to better
understand the mechanisms of An toxicity. The study of complexing
properties towards An, of series of peptides mimicking these target
proteins, is a promising way to identify the key parameters behind this
affinity and to suggest specific coordination sites of An in proteins, yet
unknown. These data are also essential to develop new highly selective
In this project, a nuclearised instrumental platform will be developed in a
laboratory dedicated to the handling of radioactive samples, to determine
the affinity scale of biomimetic peptides for An (Pu, Am, Cm…) in a single
analysis. Since the samples will be mixtures of An-peptide species at the
trace level, the strategy relies on the setting up of the simultaneous
coupling of the separation of these species by hydrophilic interaction
chromatography (HILIC), to electrospray mass spectrometry (ESIMS) and
coupled plasma mass spectrometry (ICPMS). The feasibility of the
simultaneous coupling has been previously demonstrated by the host
laboratory in non-nuclearised conditions.
The candidate will be responsible for the adaptation of the simultaneous
HILIC-ESIMS/ICPMS coupling to nuclearised instruments, taking into account
the constraints of the glove boxes as well as the technical characteristics
of each instrument. The project focuses on the implementation into glove
boxes of a nuclearisable ESI-MS and the associated chromatographic devices,
as well as the coupling of this assembly to an ICP-MS, already nuclearised.
The development of this instrumental platform requires several technical
modifications, such as the adaptation of the ionisation sources to the
nuclearisation interface, the design of specific interfaces dedicated to
electrical connections and the supply of the fluids and able to cross the
panel of the glove boxes, the autosampler modifications which must allow
its remote control etc. This nuclearisation step will be validated by the
analysis of known samples.
Afterwards, the analytical developments related to the separation
conditions of the An-peptide species by HILIC, the structural
characterization of the species by ESI-MS as well as the elemental and
isotopic characterization of the An by ICP-MS, will be carried out. The
quantitative distribution of the An thus determined in each species through
this integrated approach, will allow to establish an affinity scale in a
single analysis. Following the same approach, the selectivity of the
peptides with the highest affinity will be evaluated towards endogenous
competing ions (Ca2+, Cu2+, Zn2+, Fe3+), in the presence of the An.
*Job description:* The project will be led in collaboration between two
- LANIE (Laboratoire de développement Analytique Nucléaire, Isotopique et
Elémentaire), Service d'Etudes Analytiques et de Réactivité des Surfaces,
CEA Saclay (Host laboratory)
- CIBEST (Chimie Interface Biologie pour la Santé l’Environnement et la
Systèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SyMMES),
UMR 5819 CEA – CNRS – UGA, INAC. CEA – Direction de le Recherche
Fondamentale - Centre de Grenoble
*Qualification:* PhD in the field of instrumentation and analytical
chemistry, with a strong background in the coupling of chromatographic
techniques with different mass spectrometers. Skills in glove box handling
will be valuable.
The candidate will be in charge of the project management, covering the
nuclearisation of the instruments, the setting up of analytical
developments with nuclearised instruments, in interaction with the members
of the LANIE, the CIBEST and the different companies, the synthesis, the
interpretation and the reporting of the results as well as their
valorisation through communications and publications. The applicant is
expected to be autonomous and innovative, to demonstrate ability to write,
communicate and work in an interdisciplinary approach.
*How to apply:* To apply, send an email with detailed CV, list of
publications, and motivation letter to
[log in to unmask], DPC/SEARS/LANIE, CEA Saclay - Phone: +33
[log in to unmask], CIBEST, SyMMES, UMR 5819 CEA – CNRS – UGA, INAC,
CEA Grenoble - phone : +33 4 184.108.40.206
1 : M. Starck, F. A. Laporte, S. Oros, N. Sisommay, V. Gathu, P. L. Solari,
G. Creff, J. Roques, C. Den Auwer, C. Lebrun, P. Delangle, Chemistry – A
European Journal 23 (2017) 5281.
2 : C. Lebrun, M. Starck, V. Gathu, Y. Chenavier, P. Delangle, Chemistry –
A European Journal 20 (2014) 16566.
3 : L. Beuvier, C. Bresson, A. Nonell, L. Vio, N. Henry, V. Pichon, F.
Chartier, RSC Advances 5 (2015) 92858.
4 : E. Paredes, E. Avazeri, V. Malard, C. Vidaud, P. E. Reiller, R. Ortega,
A. Nonell, H. Isnard, F. Chartier, C. Bresson, Proceedings of the National
Academy of Sciences of the United States of America 113 (2016) 14007.
5 : E. Paredes, E. Avazeri, V. Malard, C. Vidaud, R. Ortega, A. Nonell, H.
Isnard, F. Chartier, C. Bresson, Talanta 178 (2018) 894.
Silvia Diez-Fernández, PhD.
DPC/SEARS/LANIE, CEA Saclay