I think we could be sliding down a slippery slope with 1% Triton except for the fact that Agilent by default attaches a much smaller I.D. tubing for internal standard than for sample. I don't remember the ratio of the two flows, but we ignore the default and use our own dilution plans, since to our preferences, the default setup has a liquid flow too high for our comfort zones. With a lower total liquid flow, we take better advantage of the Peltier cooled spray chamber. We do tee in internal standard at a 1:1 flow ratio, but that would imply a much lower concentration of Triton X100 in our case, not necessarily in yours if using the default setup.
I don't know how Triton X-100 would help mixing internal standard with sample, since it is viscous. They should mix in the spray chamber anyway. Maybe if your samples are proteinaceous, the intent is to better maintain the protein solubility(?). Hard to say since we don't know what your samples are. I have not committed white-white and blue-orange pump tubing i.d.s to memory yet, but I am working on it in between committing ISO 17025 and our division policy and procedures manual to memory. At my age, this is tough.
R. Steven Pappas, Ph.D.
Team Lead, Tobacco Inorganics Group
Centers for Disease Control & Prevention
4770 Buford Highway, NE
M.S. F44, Building 110
Atlanta, GA 30341-3717, USA
From: PLASMACHEM-L: Analytical Chem.(ICP's, DCP's, MIP's). [mailto:[log in to unmask]] On Behalf Of Paul Fitzgerald
Sent: Wednesday, March 29, 2017 4:40 PM
To: [log in to unmask]
Subject: Triton-X in internal standard solution
Has anyone tried adding Triton-X to the internal standard solution to improve mixing with the sample? Agilent technical support has suggested a 1% solution for the internal standards on our 7800. We are using White-White pump tubing for the sample and Blue-Orange for the internals. We’re also using the mixing block that they provide with the instrument. Thanks for your help.
Inorganic Dept. Technical Manager
449 Springbrook Rd.
Charlotte, NC 28217
704-529-6364 X 116
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