For HPLC (anion exchange) See the following group and decide whether they did something different:
VONDERHEIDE, A. P., MOUNICOU, S., MEIJA, J., HENRY, H. F., CARUSO, J. A., SHANN, J. R.,
2006: Investigation of selenium-containing root exudates of Brassica juncea using HPLC-ICP-MS
and ESI-qTOF-MS. Analyst 131, 33–40. ISSN 0003-2654.
That said, selenosulfate is catalytically hydrolyzed in presence of soft acid cation metals like CdS, CdCl2, ...
Does that give you any ideas?
R. Steven Pappas, Ph.D.
Team Lead, Tobacco Inorganics Group
Centers for Disease Control & Prevention
4770 Buford Highway, NE
M.S. F44, Building 110
Atlanta, GA 30341-3717, USA
From: PLASMACHEM-L: Analytical Chem.(ICP's, DCP's, MIP's). [mailto:[log in to unmask]] On Behalf Of Louis Wagner
Sent: Thursday, March 23, 2017 3:25 PM
To: [log in to unmask]
Subject: Selenosulfate analysis (Selenium Speciation)
I'm wondering if anyone has any experience with Selenium speciation (By HPLC-ICPMS or similar) in particular separating and quantifying Selenosulfate (SeSO4)? I'm looking for some advice on getting the chromatography to work.
I am using a Sodium Selenosulfate solution purchased from Sigma Aldrich (778354-25ML). This happens to be a mixture of Sodium Selenosulfate, Sodium sulfate and water. When I inject this on the system I get a Selenium peak that has a nearly identical retention time to Se4 yet no corresponding Sulfur peak at this retention time. There is a sulfur peak in the chromatogram but no corresponding selenium peak at that retention time. You see my problem? It looks like I'm seeing peaks for sulfate and Se4 but but nothing that might indicate selenosulfate which should have both a selenium peak and sulfur peak at the same retention times. It's as if Selenosulfate itself is not stable in my chromatographic conditions.
This is on a Agilent 7700 coupled to an Agilent bioinert LC. Column is anion exchange.
National Technical Specialist-Inorganics, Environmental Canada